Journal: Animals : an Open Access Journal from MDPI

Article Title: Variation in Follicle-Stimulating Hormone Receptor Expression Is Associated with the Twinning Rate QTL Located on Bovine Chromosome 11 in Holstein Cattle

doi: 10.3390/ani16040616

Figure Lengend Snippet: Association of LHCGR expression with twinning rate QTL genotype. Genotypes CC (green), CG (orange), and GG (purple) represent lowest, intermediate, and highest twinning rates, respectively (x-axis). The y-axis denotes ΔC T value transformed to a linear scale. Expression of LHCGR was not associated with twinning rate QTL genotype ( p = 0.18). Each genotype group is shown with individual data points and the mean ± 95% confidence interval.

Article Snippet: Assay ID , Bt03212674_m1 , Bt03213974_m1 , Bt03254301_m1.

Techniques: Expressing, Transformation Assay

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Distinct Luteinization Profiles of Cultured Human Granulosa Cells From Small Antral and Preovulatory Follicles

doi: 10.1210/clinem/dgaf218

Figure Lengend Snippet: Gene expression (RT-qPCR). (A) Granulosa cells from small antral follicles, (B) granulosa-lutein cells from preovulatory follicles. Gene expression values for the corpus luteum are shown in yellow. No significant differences were observed between control and androstenedione-treated groups. Granulosa cells from small antral follicles initially exhibit high relative expression of CYP19A1 and HSD17β1 , key genes in estrogen production, with a decline observed after 6-12 hours. In contrast, LHCGR , STAR , and CYP11A1 show an opposite trend, with increased expression after 24 to 48 hours. The relative mRNA expression of the proliferation marker MKI67 is notably higher in granulosa cells from small antral follicles compared to granulosa-lutein cells from preovulatory follicles. Granulosa-lutein cells from preovulatory follicles display a decrease in CYP19A1 and HSD17β1 expression after 12 to 24 hours, followed by an increase in HSD17β1 at 96 hours. LHCGR expression increases steadily from 12 to 96 hours, while STAR expression is initially high, decreases at 12 to 24 hours, and then stabilizes. CYP11A1 expression increases after 36 hours and continues to rise until 96 hours. Significant P values are indicated as follows: * P ≤ .05, ** P ≤ .01, *** P ≤ .001.

Article Snippet: Human LHCGR , Hs00174885_m1 , 4331182.

Techniques: Gene Expression, Quantitative RT-PCR, Control, Expressing, Marker