Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Distinct Luteinization Profiles of Cultured Human Granulosa Cells From Small Antral and Preovulatory Follicles

doi: 10.1210/clinem/dgaf218

Figure Lengend Snippet: Gene expression (RT-qPCR). (A) Granulosa cells from small antral follicles, (B) granulosa-lutein cells from preovulatory follicles. Gene expression values for the corpus luteum are shown in yellow. No significant differences were observed between control and androstenedione-treated groups. Granulosa cells from small antral follicles initially exhibit high relative expression of CYP19A1 and HSD17β1 , key genes in estrogen production, with a decline observed after 6-12 hours. In contrast, LHCGR , STAR , and CYP11A1 show an opposite trend, with increased expression after 24 to 48 hours. The relative mRNA expression of the proliferation marker MKI67 is notably higher in granulosa cells from small antral follicles compared to granulosa-lutein cells from preovulatory follicles. Granulosa-lutein cells from preovulatory follicles display a decrease in CYP19A1 and HSD17β1 expression after 12 to 24 hours, followed by an increase in HSD17β1 at 96 hours. LHCGR expression increases steadily from 12 to 96 hours, while STAR expression is initially high, decreases at 12 to 24 hours, and then stabilizes. CYP11A1 expression increases after 36 hours and continues to rise until 96 hours. Significant P values are indicated as follows: * P ≤ .05, ** P ≤ .01, *** P ≤ .001.

Article Snippet: Human LHCGR , Hs00174885_m1 , 4331182.

Techniques: Gene Expression, Quantitative RT-PCR, Control, Expressing, Marker